Device

Part:BBa_K4461508:Design

Designed by: CHIH-YU, YEH   Group: iGEM22_NYCU-Taipei   (2022-10-10)


hchA+RBS+AmilCP+double terminators


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We cloned the promoter from E.coli K-12 MG1655 by PCR. Then we used PCR again to add suffix and prefix. Also, we separated the plasmid that we want to insert the promoter into two parts. At last, we used Gibson Assembly to ligate three fragments.



Source

gene synthesis

References